|
KMID : 0613820070170040587
|
|
Journal of Life Science
2007 Volume.17 No. 4 p.587 ~ p.592
|
|
|
Current Progress in Generation of Genetically Modified Mice
|
|
Song Ki-Duk
Cho Byung-Wook
|
|
|
Abstract
|
|
|
|
Manipulation of the mouse genome by activating or inactivating the gene has contributed to the understanding of the function of the gene in the subset of cells during embryonic development or postnatal period of life. Most of all, gene targeting, which largely depends on the availability of mouse embryonic stem (ES) cells, is the milestone of development of animal models for human disease. Recombinase-mediated genome modification (Cre-LoxP and Flp-Frt etc) and the ligand-dependent regulation system, more accurate and elaborate manipulation tools, have been successfully developed and applied to dissect the mechanisms governing complex biological processes and to understand the role of protein in temporal-and spatial aspects of development. As technologies concerning refined manipulation of mouse genome are developed, they are expected to open new opportunities to better understand the diverse in vivo functions of genes.
|
|
|
KEYWORD
|
|
|
Embryonic stem cell, gene targeting, genetically modified mice, site-specific recombinase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|